ACTUATED MATRIX FOR PROTEIN IMMOBILIZATION Russian patent published in 1994 - IPC

FIELD: clinic biochemistry. SUBSTANCE: epichlorhydrin in the amount of 4 ml is added upon stirring to a suspension of 4g of Sepharose in 60 ml of sodium hydroxide. Epoxy agarose obtained is washed off from the excess of reagents on the glass filter. 10 ml of epoxy agarose obtained is added to the solution of γ-aminobutiric acid in the sodium hydroxide solution. The mixture is stirred for 2 hours at 40 C. The derivative of agarose thus obtained is washed with a hydrochloric acid and then with a distilled water. The derivative of agarose is mixed in the sodium hydroxide solution and then is washed with distilled water till neutral reaction is attained. Agarose is gradually reversed to dioxan. N-oxysuccinimid is admixed to the suspension of carboxy-agarose in 30 ml of dioxan. After complete dissolving N-oxysuccinimid a dicyclohexilcarbodiimid is added. The solvent is filtered out and washed with dioxan, methanol and adain with dioxan. EFFECT: producing of more stable and specific matrix.