METHOD FOR OBTAINING OLIGOFUROSTANOZIDES Russian patent published in 1995 - IPC

FIELD: medicine. SUBSTANCE: method involves extracting lyophilic dried cells of Dioscorea deltoidea tissue culture in water in 1:(10-20) proportion during 2-3 h. The sediment is separated by centrifuging and the extraction process repeated. Ammonium sulfate is added to the joint supernatant until complete saturation takes place and the mixture is allowed to stand during 30 min resulting in precipitation of both high and low molecular weight glycosides. The obtained precipitate is 5-6 times treated with 96% ethanol and the precipitate is separated, washed with ethanol, the supernatant liquid is fully evaporated in rotor-type vaporizer. Oligofurostanozides purity in the residue is 65% The produced preparation is repeatedly treated with several portions of 96% ethanol and the precipitate is separated and vaporized. The preparation purity is 80% Oligofurostanozides output is 50% of the theoretically expected one. EFFECT: accelerated process; increased output of the end product.