PROCESS FOR SYNTHESIS OF DEOXYTHIONUCLEOTIDE DERIVATIVES Russian patent published in 1994 - IPC

Abstract SU 1487425 A3

FIELD: molecular biology, gene engineering. SUBSTANCE: deoxythionucleotide derivatives of the general formula , wherein R₁ is 4,4-dimethoxytrityl; R₂-H is H or N=C-(CH₂)₂; B is thymine residue, N⁴ is benzoylcytosine, N⁶ is benzoyladenine and N² is isobutyrylguanidineare prepared from 5′-O-dimethoxytrityl-N-acyl-2-deoxynucleosides and S-methyl-thiodichlorophosphate used in 1.5-2-fold molar excess in pyridine at temperature of 0-5 C in presence of 4-10-fold excess of 1,2,4-triazole, and the reaction mixture, if required, is treated with ethylene cyanohydrin. Thereafter, the resulting solution is neutralized with triethyl-ammonium bicarbonate, extracted with chloroform, the extract is dried, and solvent is evaporated. The yield of the end product is up to 85-95 % . EFFECT: simplified production process.

Similar patents SU1487425A3

Title	Year	Author	Number
DERIVATIVES OF DESOXYTHIONUCLEOTIDES AS MONOMERS FOR SYNTHESIS OF DESOXYPOLYNUCLEOTIDES AND PROCESS FOR PRODUCING THE SAME	0	Kumarev Viktor Prokofevich Bogachev Viktor Semenovich Baranova Lyudmila Vasilevna	SU929647A1
METHOD OF SYNTHESIS OF 3'-PHOSPHATE,N,P-NONPROTECTED PHOSPHOTHIOATE ANALOGS OF OLIGODEOXYRIBONUCLEOTIDES	1997	Amirkhanov N.V.	RU2131881C1
METHOD FOR REMOVING CYANOETHYL BLOCKING GROUP FROM DERIVATIVES OF MONO- AND OLIGONUCLEOTIDES	0	Belikov S.I. Gorbunov Yu.A. Popov S.G.	SU809866A1
METHOD FOR P-CHLOROPHENYL ETHERS OF N, 3′-O-DIACYLDESOXYNUCLEOTIDES PRODUCTION	1988	Tsvetkov I.V. Komarova N.I. Lukhtanov E.A. Mustaev A.A. Starostin V.P.	SU1565003A1
PROCESS FOR PREPARING DEOXYNUCLEOSIDE-$$$-TRIPHOSPHATES	1994	Bogachev V.S.	RU2080325C1
DERIVATIVES OF DESOXYTHIONUCLEOTIDES AS MONOMERS FOR SYNTHESIS OF POLYTHIODESOXYNUCLEOTIDES AND PROCESS FOR PREPARING THE SAME	0	Kumarev Viktor Prokofevich Bogachev Viktor Semenovich Kobzev Viktor Fedorovich Baranova Lyudmila Vasilevna	SU910650A1
$$$-PHOSPHATE,N,P-NONPROTECTED PHOSPHOTHIOATE OLIGODEOXYNUCLEOTIDES AS PARENTAL COMPOUNDS FOR SYNTHESIS OF PHOSPHOTHIOATE OLIGONUCLEOTIDE REAGENTS FOR BIOTECHNOLOGICAL PROCESSES, PHOSPHOTHIOATE OLIGONUCLEOTIDE DERIVATIVES CONTAINING 3'- AND/OR 5'-BOUND CHEMICAL GROUPS AS PHOSPHOTHIOATE OLIGONUCLEOTIDE REAGENTS FOR BIOTECHNOLOGICAL PROCESSES AND METHOD OF THEIR SYNTHESIS	1994	Amirkhanov N.V. Zarytova V.F.	RU2069214C1
PROCESS FOR PRODUCING POLYDEZOXYNUCLEOTIDES	0	Kumarev Viktor Prokofevich Bogachev Viktor Semenovich Baranova Lyudmila Vasilevna Rivkin Mark Iosifovich	SU925964A1
METHOD OF NUCLEOSIDE LINKAGE BY 3'--5'-INTERNUCLEOTIDE SILYL UNIT	1991	Uehjs Aleksandr Ljudvik[Il] Sakha Ashiz K'Jumar[In] Khaushir Frederik German[Us]	RU2079508C1
N-PHOSPHONATES OF HYDROXYL-CONTAINING STEROIDS AS REAGENTS FOR SOLID PHASE SYNTHESIS OF STEROID DERIVATIVES OF OLIGONUCLEOTIDES OR THEIR ANALOGUES	1991	Ven'Jaminova A.G. Sergeeva Z.A. Bashirova V.Z.	RU2009147C1

SU 1 487 425 A3

Authors

Amirkhanov N.V.

Dates

1994-02-28—Published

1987-10-27—Filed