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1 489 182

(13)

A1

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IPC

(21) (22)

Application

4257446/13, 1987-05-11

(22)

Actual filing date

1987-05-11

(45)

Published

1995-10-20

(72)

Inventor

Fedosov Ju.V.Mikhajlov V.V.Zhigalina I.I.Ivanova E.P.Rasskazov V.A.Eljakov G.B.

STRAIN OF BACTERIUM ACINETOBACTER SPECIES - A PRODUCER OF ALKALINE PHOSPHATASE AND A METHOD OF ALKALINE PHOSPHATASE PREPARING Russian patent published in 1995 - IPC

Abstract SU 1489182 A1

FIELD: biotechnology. SUBSTANCE: method involves the use of the strain Acinetobacter sp. ВКПМ B-3905 40 MC as a producer. Isolation and purification of enzyme is carried out by anion-exchange and hydrophobic chromatography. Strain Acinetobacter sp. 40 MC is isolated from the celomic liquid of mussel Crenomytilus grayanus. Strain shows high specific alkaline phosphatase activity (6.5-7.1 units/mg/min) and high productivity by protein (155 mg/l cultural fluid). Specific activity of purified alkaline phosphatase is 3800-5600 units/mg/min. EFFECT: increased activity and yield of the end product, simplified method. 6 cl, 5 tbl

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SU 1 489 182 A1

Authors

Fedosov Ju.V.

Mikhajlov V.V.

Zhigalina I.I.

Ivanova E.P.

Rasskazov V.A.

Eljakov G.B.

Dates

1995-10-20—Published

1987-05-11—Filed

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