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Application

3848518/14, 1984-12-28

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Actual filing date

1984-12-28

(45)

Published

1994-03-15

(72)

Inventor

Mogutov M.A.Velikodvorskaja G.A.Piruzjan Eh.S.Mett V.L.Tsarejkina M.I.Jur'Ev M.Ju.

(73)

Holder

Institut Molekuljarnoj Genetiki Ran

RECOMBINANT PLASMID PCU201 AND A STRAIN ESCHERICHIA COLI K12 C600 PRODUCING THERMOSTABLE β-GLUCOSIDASE Russian patent published in 1994 - IPC

Abstract SU 1547312 A3

FIELD: biotechnology. SUBSTANCE: recombinant plasmid pCU201 containing gene of thermostable b-glucosidase consists of plasmid pBR322, into Bam HI-site of which DNA-fragment (size is 4.1 thousand nucleotide pairs) is cloned. This DNA-fragment obtained by partial hydrolysis of DNA from Clostridium thermocellum with restriction endonuclease Sau 3A contains gene of thermostable (at 60 C) b-glucosidase. Strain E. coli K12 C600 containing plasmid pCU201 from Collection Central Museum of Industrial Microorganisms produces thermostable b-glucosidase. EFFECT: preparing of plasmid indicated above.

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SU 1 547 312 A3

Authors

Mogutov M.A.

Velikodvorskaja G.A.

Piruzjan Eh.S.

Mett V.L.

Tsarejkina M.I.

Jur'Ev M.Ju.

Dates

1994-03-15—Published

1984-12-28—Filed

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