STRAIN OF TRANSPLANTED HUMAN MONOCYTE CELLS - A PRODUCER OF HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 Russian patent published in 1994 - IPC

FIELD: biotechnology, virology. SUBSTANCE: transplanted strain of human monocytes ЭВК-ИРА/3 - a producer of human immunodeficiency virus type 1 ( HIV-I) is obtained on the basis of human transformed monocyte cells (cellular strain-937). Cells are infected with HIV-I (from patient with AIDS) following by cloning and isolation of cell clone showing chronic infection with HIV-I. These cells produce HIV-I continuously. Obtained strain of cell-producers HIV-I is stable at cultivation (it does not require undersowing of uninfected cells, cultivation under atmosphere of CO₂, special nutrient media with expensive additives). It shows technological effectiveness and can be cultured at roller-suspension regime. Strain is deposited at N 4005 in Collection VNII of Molecular Biology and Virology Institutes. EFFECT: high and stable level of virus production, simplified method of virus cultivation. 1 tbl