METHOD FOR OBTAINING THROMBOXAN B LABELLED WITH TRITIUM Russian patent published in 1994 - IPC

Abstract SU 1732514 A1

FIELD: methods for producing biologically active compounds. SUBSTANCE: method consists in separation and partial purification of special synthetase from vesicular gland of wether and thromboxan - synthetase from human thrombocytes, incubation of [3H] arachidonic acid with concentration of 10-100 microM with partially purified prostaglandin of H-synthetase preparation with 0.3-3.3 microM of [3H] arachidonic acid per mg of special synthetase for not more than 4 min, and addition of preparation of partially purified thromboxan synthetase up to concentration up to 0.11-0.545 mg/ml and incubation for 25-40 min at 25 C. EFFECT: higher yield and quality of target product by increasing molar radioactivity. 5 dwg

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SU 1 732 514 A1

Authors

Lazurkina T.Ju.

Mjasoedov N.F.

Nagaev I.Ju.

Shevchenko V.P.

Shram S.I.

Dates

1994-03-15Published

1990-08-20Filed