FIELD: methods for producing biologically active compounds. SUBSTANCE: method consists in separation and partial purification of special synthetase from vesicular gland of wether and thromboxan - synthetase from human thrombocytes, incubation of [3H] arachidonic acid with concentration of 10-100 microM with partially purified prostaglandin of H-synthetase preparation with 0.3-3.3 microM of [3H] arachidonic acid per mg of special synthetase for not more than 4 min, and addition of preparation of partially purified thromboxan synthetase up to concentration up to 0.11-0.545 mg/ml and incubation for 25-40 min at 25 C. EFFECT: higher yield and quality of target product by increasing molar radioactivity. 5 dwg
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Authors
Dates
1994-03-15—Published
1990-08-20—Filed