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2 034 292

(13)

C1

(51)

IPC

(21) (22)

Application

5041354/25, 1992-05-08

(22)

Actual filing date

1992-05-08

(45)

Published

1995-04-30

(72)

Inventor

Mundus D.A.Grajfer D.M.Karpova G.G.

(73)

Holder

Novosibirskij Institut Bioorganicheskoj Khimii So Ran

METHOD FOR EXTRACTION OF TRANSFER RIBONUCLEIC ACID FROM PREPARATION OF TOTAL RIBONUCLEIC ACID FROM HUMAN PLACENTA Russian patent published in 1995 - IPC

Abstract RU 2034292 C1

FIELD: biotechnology. SUBSTANCE: transfer ribonucleic acid is extracted from preparation of total ribonucleic acid of human placenta by reversed-phase chromatography on coarse-pored microspheric silica gel with grafted aliphatic groups C18 with use of acetonitrile as eluent of concentration gradient. Chromatography stages are conducted in the following sequence first, preparation of total ribonucleic acid is passed through column filled by dry method; next stages, including concentration of fractions and extraction of desired product, are conducted on column filled by suspension method. In this case, before extraction of product by chromatography, concentrated fraction is subjected to aminoacylation with phenylalanine. EFFECT: reduced process duration and increased purity of desired product from 70-80 to 99%. 3 dwg

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RU 2 034 292 C1

Authors

Mundus D.A.

Grajfer D.M.

Karpova G.G.

Dates

1995-04-30—Published

1992-05-08—Filed

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