FIELD: synthesis of bioactive substance labeled with radioactive isotopes. SUBSTANCE: for synthesis of F3α-prostaglandin labeled with tritium a purified preparation of PGH-synthetase is used. The latter is incubated with labeled thymiodonic acid at the ratio 60-70 nmoles per a unit of PGH-synthetase activity at 15-60 C for 1-7 min following by addition of SnCl2 in methanol up to concentration 35-50 mM. EFFECT: increased yield of labeled F3α-prostaglandin by 1.6-1.9 times, increased radioactivity by 11%. 5 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF SYNTHESIS OF PROSTAGLANDINS TYPE e, OR THEIRS DERIVATIVES TYPE A, B, F, OR METHYL ESTERS OF PROSTAGLANDIN TYPE 1 MULTIPLE LABELED WITH TRITIUM | 1988 |
|
SU1646252A3 |
| METHOD OF PREPARING OF THROMBOXANE B LABELED WITH TRITIUM | 1990 |
|
SU1732513A3 |
| METHOD OF PROSTAGLANDIN F3α PREPARING | 1990 |
|
RU1774659C |
| METHOD FOR OBTAINING THROMBOXAN B LABELLED WITH TRITIUM | 1990 |
|
SU1732514A1 |
| 15-HYDROXY-5Z,8Z,11Z,13E-EICOZATETRAENIC ACID MULTIPLE LABELED WITH TRITIUM AT DOUBLE BONDS AND METHOD OF ITS SYNTHESIS | 1993 |
|
RU2073665C1 |
| METHOD OF PREPARING QUATERNARY AMINE SALTS CONTAINING TRITIUM LABELLED AROMATIC RADICAL AT NITROGEN ATOM | 1995 |
|
RU2089532C1 |
| METHOD OF SYNTHESIS OF HEXAPEPTIDE AMINO ACID FRAGMENTS MULTIPLE-LABELED WITH TRITIUM AT α-POSITION | 1988 |
|
SU1736126A3 |
| METHOD OF SYNTHESIS OF UNSATURATED SIMPLE LIPIDS MULTIPLE-LABELED WITH TRITIUM AT DOUBLE BONDS | 1988 |
|
SU1582553A3 |
| 0 |
|
SU1631942A1 | |
| [H]-7-CHLORO-1,3-DIHYDRO-1-METHYL-5-PHENYL-2H-1,4- BENZDIAZEPINE-2-ONE HIGH-LABELED WITH TRITIUM AND METHOD FOR ITS PREPARING | 2003 |
|
RU2247116C2 |
