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1 774 534

(13)

A1

(51)

IPC

(21) (22)

Application

4755643/13, 1989-11-03

(22)

Actual filing date

1989-11-03

(45)

Published

1995-08-20

(72)

Inventor

Usachev N.A.Solomin A.A.Isangalin F.Sh.Chugunov V.A.

METHOD FOR PRODUCING EXOTOXIN-CONTAINING PREPARATION FROM BACILLUS THURINGIENSIS CULTURE LIQUID Russian patent published in 1995 - IPC

Abstract SU 1774534 A1

FIELD: microbiology. SUBSTANCE: method involves concentrating culture liquid on ultrafilters and diafiltering the obtained concentrate with water. Barium acetate solution is added to the filter at the rate of at least 3 g per 1 g of the exotoxin at pH 8.0-8.5. Barium salt of the exotoxin is separated on the ultrafilter and diafiltered with ammonia solution with pH 3.5-10. Ammonium carbonate is added to the concentrate to solve the sediment. The resulting solution is repeatedly condensed on the ultrafilter at 58-80 C, diafiltered with water and treated by means of lyophilic drying. EFFECT: higher contents of the preparation in the end product. 9 tbl

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SU 1 774 534 A1

Authors

Usachev N.A.

Solomin A.A.

Isangalin F.Sh.

Chugunov V.A.

Dates

1995-08-20—Published

1989-11-03—Filed

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