NUCLEIC ACID FRAGMENT ENCODING HUMAN PROAPOLIPOPROTEIN A-1 Russian patent published in 1994 - IPC

FIELD: genetic engineering. SUBSTANCE: sequence of recombinant DNA is prepared involving sequence, in which part of natural encoding sequence is replaced with DNA- fragment. This fragment encodes the same amino acids but it consists of another nucleotide sequence. As a result, formation of "pin"-like structures is decreased or prevented. Sequence encoding amino acids from -6 to +14 in proapo A-1 is replaced with the following one: 5'-ATGAGACATTTCTGGCAGCAGGAACCTCCACAATCTCCTTGGGATAGAGTTAAGGACTTG-3', which involves additional ATG-codon of translation initiation and modifying codons of amino acid groups: C -1, +1, +3, +4, +5, +6, +7, +10, +11, and +14. Obtained DNA-fragment has a sequence encoding human proapolipoprotein A-1, which is used for transformation of recipient strains for producing of human proapolipoprotein A-1. EFFECT: preparing of DNA-fragment indicated above.