PROCESS FOR PRODUCING ALCOHOLOXYDASE OF HIGHER ALCOHOLS Russian patent published in 1994 - IPC

FIELD: biotechnology. SUBSTANCE: essence of this process resides in that enzyme is prepared by successively fractionating solubilized microsomal fraction of paraffin-acidifying yeast with polyethyleneglycol 6000 at its final concentration of 6 to 8 % in presence of 0.8 to 1.2 % sodium cholate and 0.08 to 0.12 % Triton X-100 and then with hydropol 294 at its final concentration of 8 to 10 % in presence of 0.8 to 1.2 % sodium cholate and 0.8 to 1.2 % Triton X-100 and then salting out desired product from polymer phase using ammonium sulfate at its final concentration of 18 to 20 % of saturation level. Whenever it proves necessary to obtain chromatographically homogeneous preparation, end product must be subjected to chromatography with hydroxylapatite in 10 to 300 mM gradient of potassium-phosphate buffer at pH value of 6.8. EFFECT: more advanced technology. 2 cl, 3 tbl