METHOD OF LIGNINOLYTIC ENZYMES PREPARING Russian patent published in 1994 - IPC

FIELD: biotechnology. SUBSTANCE: strain of white mold fungus Panus tigrinus 8/18 is grown under submerged conditions on synthetic medium containing maltose, nitrogen and phosphorus sources, mineral salts, thiamine, trace elements in the presence of inductor, Tween-80 and increased concentration of manganese. At the process of cultivation temperature increase is carried out at the range of temperatures for optimal culture growth. For process carrying out mycelium immobilized on the polycaproamide fiber can be used. Method provides to carry out effective process of lignolytic enzymes preparing showing broad spectrum action, for example, Mn-dependent peroxidase with functions of "key" enzyme, and oxidase catalyzing oxidation of nonphenolic lignin substructures. Lignolytic enzymes can be used in paper and pulp, wood working, chemical industry and in agriculture. EFFECT: improved method of enzymes preparing. 2 cl, 1 tbl