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2 394 912

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IPC

C12N15/53(2006-01-01)

C12N15/11(2006-01-01)

C12N15/63(2006-01-01)

C12N1/15(2006-01-01)

C12N9/02(2006-01-01)

(21) (22)

Application

2008123732/13, 2008-06-17

(24)

Start date

2008-06-17

(22)

Actual filing date

2008-06-17

(45)

Published

2010-07-20

(72)

Inventor

Koroleva Ol'Ga VladimirovnaStepanova Elena VladimirovnaFedorova Tat'Jana Vasil'EvnaLoginov Dmitrij SergeevichCherkashin Evgenij AleksandrovichBenevolenskij Sergej VladimirovichVavilova Ekaterina AleksandrovnaChulkin Andrej MikhajlovichAbjanova Al'Fija RamilevnaBudarina Zhanna IgorevnaJurkova Tat'Jana VladimirovnaZakharova Marina ViktorovnaLeont'Evskij Aleksej Arkad'EvichSolonin Aleksandr SergeevichPozhidaeva Zoja Aleksandrovna

(73)

Holder

Institut Biokhimii Im. A.N. Bakha Rossijskoj Akademii NaukInstitut Biokhimii I Fiziologii Mikroorganizmov Im. K. Skrjabina Rossijskoj Akademii Nauk

RECOMBINANT LACCASE OF LIGNINOLYTIC FUNGUS Trametes sp AND METHOD OF ITS OBTAINING Russian patent published in 2010 - IPC C12N15/53 C12N15/11 C12N15/63 C12N1/15 C12N9/02

Abstract RU 2394912 C2

FIELD: medicine.

SUBSTANCE: plasmid contains a DNA fragment encoding laccase C1 of ligninolytic fungus Trametes hirsuta or a DNA fragment hybridised with SEQ ID N0:1 in tough conditions, under control of promoter functioning in this cell. The resulting cell is a producer of laccase C1. The invention also refers to method of laccase obtaining using to the indicated cell.

EFFECT: obtaining laccase with high catalytic activity.

6 cl, 3 dwg, 1 tbl, 5 ex

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RU 2 394 912 C2

Authors

Koroleva Ol'Ga Vladimirovna

Stepanova Elena Vladimirovna

Fedorova Tat'Jana Vasil'Evna

Loginov Dmitrij Sergeevich

Cherkashin Evgenij Aleksandrovich

Benevolenskij Sergej Vladimirovich

Vavilova Ekaterina Aleksandrovna

Chulkin Andrej Mikhajlovich

Abjanova Al'Fija Ramilevna

Budarina Zhanna Igorevna

Jurkova Tat'Jana Vladimirovna

Zakharova Marina Viktorovna

Leont'Evskij Aleksej Arkad'Evich

Solonin Aleksandr Sergeevich

Pozhidaeva Zoja Aleksandrovna

Dates

2010-07-20—Published

2008-06-17—Filed