FIELD: medicine. SUBSTANCE: finding out antibodies on surface of lymphocyte producing antibodies. For that purpose lymphocytes to be investigated are incubated with specific antigen. Cells are fixed by acetone on subject glasses after forming complexes. Then endogenous peroxidase activity is inhibited by means of hydrogen peroxide, after that incubation is carried out with conjugate and substrate in succession. Reaction is considered positive if lymphocyte membrane receives brown color. The method enables to determinate production of antibodies by single cells. It is impossible to define this low level of immoglobulin by means of well known immunofermental hard-phasic analysis. EFFECT: improved accuracy of method.
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