METHOD OF PRODUCING PREPARATION HAVING PROPERTIES OF BONE MARROW Russian patent published in 1995 - IPC

FIELD: biology. SUBSTANCE: coagulated blood is washed against plasma by physiologic salt solution, poured by 5 % solution of sodium citrate, allowed to stand within about 12 h and is then centrifuged. After complete hemolysis water is added into thus prepared precipitate and additional centrifugation is carried out. Hemolyzate is separated and poured by acetone. Thus prepared mixture is centrifuged, liquid is separated and precipitate being washed by acetone several times. Then centrifugation, filtration and drying are carried out. Thus prepared dry powder is cherry-colored. Modification of proposed preparation which has no antigen properties also may be prepared. Said process is carried out by adding concentrated hydrochloric acid into precipitate before its filtration and drying. Quantity of said acid is 0.1 of volume of acetone. Thus prepared mixture is allowed to stand within few minutes and then it is centrifuged. Thus prepared precipitate is washed several times by acetone, filtered and dried. Desired powder has slight grey color. EFFECT: improves efficiency of the method. 2 cl