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2 039 823

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C1

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IPC

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Application

5057016/13, 1992-07-29

(22)

Actual filing date

1992-07-29

(45)

Published

1995-07-20

(72)

Inventor

Denisov A.A.Torskij S.P.Nikolaeva O.G.Volozhantseva I.Ja.Urakov N.N.Korobko V.G.

(73)

Holder

Gosudarstvennyj Nauchno-Issledovatel'Skij Institut Prikladnoj Mikrobiologii

METHOD FOR MANUFACTURING HUMAN RECOMBINATION LYMPHOTOXIN Russian patent published in 1995 - IPC

Abstract RU 2039823 C1

FIELD: medicine; biological engineering. SUBSTANCE: essence of this process resides in cultivating E. coli VKPM-5279 lymphotoxin strain-producer prior to its direct application at temperature of 30 to 32 C during 20 to 24 h in broth containing 20 mcg/cu. cm of chloramphenicol, destroying cells by ultrasonic treatment, separating cellular residue, purifying recombination lymphotoxin derived from super-precipitant liquid by method of gel-filtration employing sedefax G=150 and ion-exchange chromatography using DEAE-TSK 250 F=gel and CM-sedefax A=25. EFFECT: more sophisticated technique. 1 dwg, 5 tbl

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RU 2 039 823 C1

Authors

Denisov A.A.

Torskij S.P.

Nikolaeva O.G.

Volozhantseva I.Ja.

Urakov N.N.

Korobko V.G.

Dates

1995-07-20—Published

1992-07-29—Filed

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