FIELD: medicine; biological engineering. SUBSTANCE: essence of this process resides in cultivating E. coli VKPM-5279 lymphotoxin strain-producer prior to its direct application at temperature of 30 to 32 C during 20 to 24 h in broth containing 20 mcg/cu. cm of chloramphenicol, destroying cells by ultrasonic treatment, separating cellular residue, purifying recombination lymphotoxin derived from super-precipitant liquid by method of gel-filtration employing sedefax G=150 and ion-exchange chromatography using DEAE-TSK 250 F=gel and CM-sedefax A=25. EFFECT: more sophisticated technique. 1 dwg, 5 tbl
Authors
Dates
1995-07-20—Published
1992-07-29—Filed