FIELD: medicine. SUBSTANCE: method involves isolation of fraction showing maximal NADH-oxidase activity due to increased purification degree of the end product. Source raw: nonconditioned, preliminary used low-mineralized slime sulfide silt which were decalcined and desulfurized with 2M hydrochloric acid solution, washed with water, extracted with 0.5M sodium hydroxide solution, filtered, precipitated with 50% sulfuric acid solution. Precipitate is washed with water, ethanol, dissolved in 0.02M sodium hydroxide solution followed by repeated precipitation. Precipitate washed with water is dissolved in 0.02M sodium hydroxide solution is passed through cationite in H+-form and dried. EFFECT: improved method of preparation preparing.
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Authors
Dates
1995-09-10—Published
1992-07-20—Filed