PELOID HYMATOMELANIC ACID ANALYSIS METHOD Russian patent published in 2008 - IPC G01N33/15 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to medical technology and pharmacology, and can be used for standardisation of biologically active substance based on peloid hymatomelanic acids. Method includes spectrophotometric analysis of hymatomelanic acids solutions. Hymatomelanic acid batch is processed using 0.02 M sodium hydroxide solution within 2 hours in water bath, and then pH value of solution is reduced to 7.4. Prior to spectrophotometric analysis tested sample is diluted with distilled water, and optical density of solution is measured within value range 310-600 nm. Thus for qualitative identification purpose analysis is performed at absorption maxima 350 and 380 nm, and for quantitative evaluation of humic acids analysis is performed at wavelength 380 nm using calibration diagram. Invention allows for qualitative and quantitative analysis of substance based on humic acids within 15 minutes, not considering time of sample preparation leaving 2.5-3.0 hours.

EFFECT: method is characterised with lower costs, lower efforts, higher accuracy, sensitivity and reproducibility.

2 tbl, 2 dwg