STRAIN OF HYBRID CULTURED MURINE CELLS MUS MUSCULUS L USED FOR PREPARING MONOCLONAL ANTIBODIES TO DIGOXIN Russian patent published in 1995 - IPC

Abstract RU 2049818 C1

FIELD: clinical biotechnology. SUBSTANCE: method involves preparing strain of hybrid cultured murine cells Mus musculus which produces monoclonal antibodies to digoxin. Strain D-22 is prepared by hybridization of murine splenocytes of line BALB/c with myeloma X63 Ag.865.3 cells. Monoclonal antibodies are referred to subclass IgG1, affinity constant of antibodies is 5·10⁹M^-1. Monoclonal antibodies specifically bind with digoxin, their crossed reactivity with digoxin analogs is 8.9% EFFECT: preparing cell strain indicated above. 2 dwg, 1 tbl

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RU 2 049 818 C1

Authors

Danilova N.P.

Vasilov R.G.

Dates

1995-12-10—Published

1993-04-05—Filed