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2 381 271

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(51)

IPC

C12N5/16(2006-01-01)

(21) (22)

Application

2008127785/13, 2008-07-10

(24)

Start date

2008-07-10

(22)

Actual filing date

2008-07-10

(45)

Published

2010-02-10

(72)

Inventor

Vetchinin Sergej SergeevichBelova Elena ValentinovnaBaranov Andrej MitrofanovichSapozhnikov Aleksandr MikhajlovichMargulis Boris Aleksandrovich

(73)

Holder

Federal'Noe Gosudarstvennoe Uchrezhdenie Nauki Gosudarstvennyj Nauchnyj Tsentr Prikladnoj Mikrobiologii I Biotekhnologii Gnts Pmb)Institut Bioorganicheskoj Khimii Im. Akademikov M.M. Shemjakina I Ju.A. Ovchinnikova Rossijskoj Akademii Nauk Ran)

STRAIN OF HYBRID CULTIVATED ANIMAL CELLS Mus museums 2E4-PRODUCER OF MONOCLONAL ANTIBODIES SPECIFIC TO HEAT SHOCK PROTEIN 70 Russian patent published in 2010 - IPC C12N5/16

Abstract RU 2381271 C1

FIELD: medicine.

SUBSTANCE: invention refers to biotechnology. Hybridoma strain is prepared by immunisation of BALB/c mice with bovine HSP 70 within 78 days. On the third day, splenocyte hybridisation of immune mice (10 cells) with murine myeloma cells P3-X63 Ag/8-653 (10⁷ cells) is carried out. As a fusion agent, polyethylene glycol of molecular weight 4000 (Merk, Germany) is applied. Hybridisation is followed with selection, screening, cloning and cryopreservation of hybridoma.

EFFECT: invention can be used for preparing monoclonal antibodies (MCA) to heat shock protein 70 (HSP 70).

4 dwg, 1 tbl, 6 ex

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RU 2 381 271 C1

Authors

Vetchinin Sergej Sergeevich

Belova Elena Valentinovna

Baranov Andrej Mitrofanovich

Sapozhnikov Aleksandr Mikhajlovich

Margulis Boris Aleksandrovich

Dates

2010-02-10—Published

2008-07-10—Filed