FIELD: medicine.
SUBSTANCE: invention can be used for production of monoclonal antibodies (MCAs) to heat shock protein 70 (HSP 70). A hybridoma strain is made by immunisation of BALB/c mice with bovine HSP 70 within 78 days. For the third days, splenocytes of immune mice (108 cells) are hybridised with murine myeloma cells P3-X63 Ag/8-653 (107 cells). A fusion agent is polyethylene glycol of molecular weight 4000 (Merk, Germany). The hybridisation is followed with selection, screening, cloning and cryopreservation of hybridoma. Hybridoma 6G2 is deposited in the microorganism collections of "ГНТТ ПМБ" under No. H-2. MCA.
EFFECT: produced hybridoma under the invention is more evident to be detected as HSP 70 on the cell surfaces, and change of endocellular HSP 70 level when exposed to the stress factors.
4 dwg, 1 tbl, 6 ex
| Title | Year | Author | Number |
|---|---|---|---|
| STRAIN OF HYBRID CULTIVATED ANIMAL CELLS Mus museums 2E4-PRODUCER OF MONOCLONAL ANTIBODIES SPECIFIC TO HEAT SHOCK PROTEIN 70 | 2008 |
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| MUS MUSCULUS CULTIVATED HYBRID CELL STRAIN PRODUCER OF MONOCLONAL ANTIBODIES SPECIFIC TO HUMAN PROTEIN C (VERSIONS) | 2010 |
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| STRAIN OF HYBRIDOUS CULTURED CELLS MUS MUSCULUS, USED FOR PREPARATION OF MONOCLONAL ANTIBODIES TO THE HUMAN GLIAL FIBRILLAR ACID PROTEIN | 0 |
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| CULTIVATED HYBRID CELL STRAIN OF MOUSE Sp2/0Ag14-SpBcG/APC-15/A3 - PRODUCER OF MONOCLONAL ANTIBODIES SPECIFIC TO HUMAN PROTEIN C, MONOCLONAL ANTIBODY SPECIFIC TO HUMAN PROTEIN C AND IMMUNOSORBENT FOR HUMAN PROTEIN C SORPTION CONTAINING MONOCLONAL ANTIBODY | 2011 |
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| STRAIN OF CULTIVATED HYBRID CELLS OF ANIMAL MUS MUSCULUS L CCHFV Vd-2-PRODUCER OF MONOCLONAL ANTIBODY 1E2/E5 TO VIRUS OF CRIMEAN-CONGO HAEMORRHAGIC FEVER | 2013 |
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| STRAIN OF HYBRID CULTURED MAMMALIAN CELLS MUS MUSCULUS L - A PRODUCER OF MONOCLONAL ANTIBODIES SHOWING SPECIFICITY TO BRUCELLA PROTEIN PREPARATION OF MOLECULAR MASS 18 AND 38 KDA | 1996 |
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