FIELD: medicine.
SUBSTANCE: invention can be used for production of monoclonal antibodies (MCAs) to heat shock protein 70 (HSP 70). A hybridoma strain is made by immunisation of BALB/c mice with bovine HSP 70 within 78 days. For the third days, splenocytes of immune mice (108 cells) are hybridised with murine myeloma cells P3-X63 Ag/8-653 (107 cells). A fusion agent is polyethylene glycol of molecular weight 4000 (Merk, Germany). The hybridisation is followed with selection, screening, cloning and cryopreservation of hybridoma. Hybridoma 6G2 is deposited in the microorganism collections of "ГНТТ ПМБ" under No. H-2. MCA.
EFFECT: produced hybridoma under the invention is more evident to be detected as HSP 70 on the cell surfaces, and change of endocellular HSP 70 level when exposed to the stress factors.
4 dwg, 1 tbl, 6 ex
| Title | Year | Author | Number |
|---|---|---|---|
| STRAIN OF HYBRID CULTIVATED ANIMAL CELLS Mus museums 2E4-PRODUCER OF MONOCLONAL ANTIBODIES SPECIFIC TO HEAT SHOCK PROTEIN 70 | 2008 |
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| STRAIN OF HYBRID CULTURED ANIMAL CELLS Mus musculus 2F9-PRODUCER OF MONOCLONAL ANTIBODIES SPECIFIC FOR LYSOSTAPHIN AND INHIBITING ITS LYTIC ACTIVITY | 2013 |
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| STRAIN OF HYBRIDOUS CULTURED CELLS MUS MUSCULUS, USED FOR PREPARATION OF MONOCLONAL ANTIBODIES TO THE HUMAN GLIAL FIBRILLAR ACID PROTEIN | 0 |
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| STRAIN OF HYBRID CELLS OF ANIMALS Mus musculus 3F11 - PRODUCER OF MONOCLONAL ANTIBODIES SPECIFIC TO BOTULINUS TOXIN TYPE B | 2014 |
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| STRAIN OF HYBRID CULTIVATED CELLS OF ANIMALS Mus Musculus, PRODUCING MONOCLONAL ANTIBODIES, SPECIFIC TO PEPTIDE, WHICH POSSESSES APOPTOTIC ACTIVITY WITH RESPECT TO CANCER CELLS | 2009 |
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| STRAIN OF CULTIVATED HYBRID CELLS OF ANIMAL MUS MUSCULUS L CCHFV Vd-2-PRODUCER OF MONOCLONAL ANTIBODY 1E2/E5 TO VIRUS OF CRIMEAN-CONGO HAEMORRHAGIC FEVER | 2013 |
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| MUS MUSCULUS CULTIVATED HYBRID CELL STRAIN 11D6 PRODUCER OF MONOCLONAL ANTIBODIES SPECIFIC TO LIPOPOLYSACCHARIDE FRANCISELLA TULARENSIS | 2010 |
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| STRAIN OF CULTIVATED HYBRID CELLS OF ANIMAL MUS MUSCULUS L CCHFV Vd-3-PRODUCER OF MONOCLONAL ANTIBODY 3H6/F2 TO VIRUS OF CRIMEAN-CONGO HAEMORRHAGIC FEVER | 2013 |
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| STRAIN OF CULTIVATED HYBRID ANIMAL CELLS MUS MUSCULUS L CCHFV VD-1-PRODUCER OF MONOCLONAL ANTIBODY 4G/B TO VIRUS OF CRIMEAN-CONGO HEMORRHAGIC FEVER (CCHF) | 2013 |
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