FIELD: biology. SUBSTANCE: desired ferment is prepared of leech Hirudo medicinalis. Extract of homogenate of said leech is fractionated by ammonia sulfate. Fraction having 40-60 saturation is purified with the help of anion exchange and cation exchange resins. The processes are carried out in 0.02 M tris-HCl and 0.02 M N-morpholine ethane sulfonic acid respectively, pH being 7.5 and 5.5 respectively. Then high effective column gel filtration is carried out. Molecular weight of desired ferment is 15000 ± 1000 Dal, its isoelectric point is 7,3 ± 0,1. Desired ferment enables to hydrolyze isopeptide bonds in γ = Glu = ζ = Lys-Glu-γ = Glu = pNa-Lys and amide bond in γ = Glu = pNa-Glu-pNa substrate, both processes are carried out at pH 8.0. Iodoacetamide, iodoacetate inhibits stability of desired ferment at their concentrations not lower 10-2M. Michaelis constant of desired ferment is 2,9·10-5M in γ = Glu = pNa-Glu-pNa substrate. EFFECT: improves quality of desired product.
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