FIELD: medicine, biochemistry. SUBSTANCE: invention relates to new thrombolytic enzyme destabilase, endo-ε(γ-Glu)-Lys-isopeptidase, showing exo-ε(γ-Glu)-Lys-isopeptidase and amidolytic activity. Enzyme is obtained from leech of family Hirudinidae. Leech extract is subjected for affinity chromatography, purified additionally by ion-exchange chromatography, desalted by gel-filtration and protein of molecular mass 126 000 ± 300 Da is collected. Partial amino acid sequence of this enzyme is identified. Product can be used in medicine for thrombosis therapy and in experimental medicine. EFFECT: new enzyme indicated above, improved method of preparing. 2 dwg
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1991 |
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RU2049819C1 |
| PEPTIDES SHOWING ANTISTRESS, ANTICONVULSANT AND NEUROPROTECTIVE EFFECT |
1997 |
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1997 |
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| METHOD OF SYNTHESIS OF δ-SLEEP PEPTIDE |
1995 |
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| RECOMBINANT PLASMID DNA pET15/N-Dest+ AND METHOD FOR THE PREPARATION, THE STRAIN OF ESCHERICHIA COLI BL21(DE3)GOLD, TRANSFORMED BY RECOMBINANT DNA pET15/N-Dest+, AND A METHOD OF ITS RECEPTION, A METHOD FOR OBTAINING RECOMBINANT BIFUNCTION ENZYME - DESTABILASE OF MEDICAL LEECH |
2015 |
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1994 |
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1988 |
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| METHOD OF SYNTHESIS OF SECONDARY AMINES |
1998 |
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| RECOMBINANT PLASMID DNA PPINS07 ENCODING FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN AND STRAIN OF BACTERIUM ESCHERICHIA COLI - PRODUCER OF FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN |
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