FIELD: biotechnology. SUBSTANCE: method involves the use of 25-30% suspension of rabbit liver dead after viral hemorrhagic disease prepared on the buffered solution, pH 7.2, Virus is extracted twice for 20-48 hr at 6 ± 2 C, at periodic stirring and filtration through the sterile cotton-gauze filter. Inactivation of viral raw is carried out at 60 C for 5 hr. Material is stabilized by addition of 5% lactose and 1% gelatin at the ratio 1:1 and stabilizing agent followed by lyophilization. EFFECT: improved method of vaccine preparing. 4 tbl
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Authors
Dates
1996-02-20—Published
1992-08-17—Filed