FIELD: biotechnology. SUBSTANCE: product: 18-carboxy-20-(carboxymethyl)-8-ethenyl-13-ethyl-2,3-dihydro-3,7,12,17- tetramethyl-21H, 23H-porphyn-2-propionic acid or its salts. Chlorine e6 is prepared using cyanobacterium biomass as a raw, for example, of genus Spirulina prepared by growing under condition of artificial or solar illumination on nutrient media at pH 8.0-12.0 and with preliminary treatment of biomass with spirituous alkali solution. Prepared spirituous extract of chlorophyll is treated with acid and pheophytin is obtained. Pheophytin is washed with nonpolar solvent, for example, hexane and subjected for saponification up to the end product formation which is precipitated with hydrochloric acid. EFFECT: improved method of preparing. 5 cl
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Authors
Dates
1996-02-20—Published
1993-07-14—Filed