PHOTOSENSIBILIZATOR AND METHOD FOR ITS PREPARING Russian patent published in 2006 - IPC A61K31/409 A61K47/26 A61K47/30 A61P35/00 

FIELD: medicine, oncology, pharmacology.

SUBSTANCE: invention relates to methods for preparing biologically active substances. Method for preparing a photosensitizer involves treatment of spirulina preliminary dried by lyophilization method in methanol for preparing a precipitate containing chlorin e₆ followed by its washing out, suspending and repeated centrifugation. Prepared precipitate is dissolved in aqueous solution of N-methyl-D-glucamine, pH value is brought about to 7.75-8.20, then 510-520 ml of apyrogenic water is added to obtain the optical density of solution 200-210 U at 654 nm and pH = 9.0 followed by filtration of solution through filter with pores size 0.22 mmc and polyvinylpyrrolidone of molecular mass from 9600 to 11500 Da is added. Prepared photosensitizer for photodynamic therapy comprises chlorin e₆ in the amount 98%, not less, N-methyl-D-glucamine in the mole ratio = 1:2 and polyvinylpyrrolidone of molecular mass from 9600 to 11500 Da wherein chlorin e₆ has the following structural formula: . Invention provides the development of a new medicinal formulation possessing the more effective therapeutic properties for treatment of different oncological diseases by methods of photodynamic therapy based on the higher content of chlorin e₆. Invention can be used for preparing a water-soluble form of highly purified chlorin e₆ that can be used as a photosensitizer in photodynamic therapy of cancer and other neoplasm of different genesis.

EFFECT: improved preparing method, valuable medicinal properties of agent.

5 cl, 8 dwg, 3 ex