METHOD OF IMMOBILIZED ENZYME PREPARING Russian patent published in 1996 - IPC

FIELD: biochemistry, enzymology. SUBSTANCE: organic carrier - hemosorption fiber made on the basis of copolymer of acrylonitrile with 2,5-vinylpyridine is treated with aqueous solutions of different enzymes - alkaline phosphatase, peroxidase, papain, α-chymotrypsin. Enzyme quantity is 1.2-17 mg/g adsorbed on the fibers is 1.2-17 mg/g and depends on the enzyme nature, its concentration in solution and time adsorption. Activity of immobilized enzymes is 45-75% of native value and depends on the nature of applied enzyme and used substrate. Time of half-inactivation is 14 days and above. EFFECT: high activity and stability of immobilized enzymes, decreased cost of carrier, simplified procedure.