FIELD: biotechnology. SUBSTANCE: method involves the preparing polypeptide or glycoproteide showing activity of human factor that stimulates the formation of granulocyte colonies. Method involves the preparing double-stranded cDNA which is complementary to messenger RNA (mRNA) encoding polypeptide indicated above and human chromosomal gene encoding polypeptide that shows factor activity stimulating granulocyte colony formation, gene insertion into vector, transformation of prepared recombinant DNA of recipient cells, isolation and purification of the end product. EFFECT: improved method of stimulating factor preparing. 40 dwg, 17 tbl
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Year |
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1998 |
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1999 |
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|
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1998 |
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|
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| METHOD AND SET FOR DETECTING GENE ENCODING MEMBRANE-BOUND PROTEIN, VECTOR (VARIANTS) |
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RU2246538C2 |
