FIELD: molecular biology, biotechnology, genetic engineering.
SUBSTANCE: invention can be used for detection and isolation of DNA encoding peptide that elicits the secretory activity. DNA encoding human protein mpI without signal sequence is ligated in vector with testing cDNA encoding this peptide. Chimeric gene is expressed in cells for evaluating the capacity for cellular proliferation. The presence of signal peptide in testing cDNA is assayed by capacity for proliferation. DNAs encoding secretory proteins are detected by construction of cDNA library and the following its screening. Invention provides detection of secretory proteins, among them, membrane proteins of the I-st and the II-d type.
EFFECT: improved assay and isolating methods.
11 cl, 1 dwg, 5 tbl, 3 ex
| Title |
Year |
Author |
Number |
| METHOD AND SET FOR DETECTING GENE ENCODING MEMBRANE-BOUND PROTEIN, VECTOR (VARIANTS) |
1999 |
- Tsutija Masajuki
- Saito Mikiesi
- Okhtomo Tosikhiko
|
RU2246538C2 |
| POLYPEPTIDE-LIGAND FLT3 (VARIANTS), POLYNUCLEOTIDE (VARIANTS), EXPRESSING VECTOR (VARIANTS), STRAIN OF CELL CHO (VARIANTS), METHOD FOR PRODUCING POLYPEPTIDE-LIGAND FLT3 (VARIANTS), PHARMACEUTICAL COMPOSITION (VARIANTS) |
1994 |
- Lajmehn Stjuart D.
- Bekmann M. Patritsija
|
RU2220979C2 |
| MODIFIED SIGNAL PEPTIDE OF ESCHERICHIA COLI ENTEROTOXIN-II AND MICROORGANISM EXPRESSING FUSION PROTEIN OF MENTIONED PEPTIDE WITH HETEROLOGICAL PROTEIN |
1999 |
- Kvon Se Chang
- Dzung Sung Joub
- Skhin Khoon
- Choj Dzaj Do
- Choj Ki Doo
- Li Gvan Sun
|
RU2198179C2 |
| THROMBOPOIETIN |
1994 |
- Iton Dehn L.
- De Sovazh Frederik Zh.
|
RU2245365C2 |
| METHOD OF HETEROLOGICAL PROTEIN PRODUCING |
1994 |
- Lars Kristiansen
- Ens G. Litske Petersen
|
RU2143495C1 |
| INTRACELLULAR DOMAIN OF PROTEIN HER-2/NEU FOR PROPHYLAXIS AND TREATMENT OF MALIGNANCY |
1996 |
- Chiver Martin Eh.
- Disis Mehri L.
|
RU2236461C2 |
| NEW LIGAND OF CYTOKINE RECEPTOR ZCYTOR17 |
2003 |
- Spresher Sindi A.
- Kejper Jozef L.
- Dasovich Marija M.
- Grant Frehnsis Dzh.
- Khehmmond Andzhela K.
- Novak Dzhulija E.
- Gross Dzhejn A.
- Dillon Stejsi R.
|
RU2490276C2 |
| NEW CYTOKINE ZALPHA 11-LIGAND |
2000 |
- Novak Dzhulija Eh.
- Presnell Skott R.
- Spreker Sindi A.
- Foster Donald K.
- Kholli Richard D.
- Gross Dzhejn A.
- Dzhonston Dzhehnet V.
- Nelson Ehndrju Dzh.
- Dillon Stejsi R.
- Khehmmond Andzhela K.
|
RU2258710C2 |
| DNA MOLECULE FOR EXPRESSION OF BILE SALT-STIMULATED LIPASE |
1996 |
|
RU2157847C2 |
| HEMOPOIETIC PROTEIN (VARIANTS), DNA SEQUENCE, METHOD FOR PREPARING HEMOPOIETIC PROTEIN, METHOD FOR SELECTIVE EXPANSION OF HEMOPOIETIC CELLS, METHOD FOR PRODUCING DENDRITIC CELLS, METHOD FOR STIMULATING FOR PRODUCING HEMOPOIETIC CELLS IN PATIENT, PHARMACEUTICAL COMPOSITION, METHOD FOR TREATMENT |
1997 |
- Mak Verter Charl'Z A.
- Feng Jikving
- Mak Kern Dzhon P.
- Sammers Nina L.
- Stehjten Nikolas R.
- Striter Filip R.
- Minnerli Dzhon K.
- Minster Nehnsi I.
- Vul'F S'Juzn L.
|
RU2245887C2 |
