METHOD OF MUTANT PROTEASE PREPARING Russian patent published in 1996 - IPC

FIELD: genetic engineering. SUBSTANCE: invention relates to preparing mutant proteases in cells of Bacillus. Method involves preparing gene encoding protease with amino acid sequence which shows at least 70% homology with that of serine protease PB82 and containing amino acid substitution at positions 32 and/or 33, and/or 48, and/or 49, and/or 50, and/or 51, and or 52, and/or 53, and/or 54, and/or 58, and/or 59-62, and/or 94-107, and/or 116-118, and/or 123-134, and/or 150, and/or 152-156, and/or 158-161, and/or 164, and/or 166, and/or 169, and/or 175-186, and/or 197, and/or 198, and/or 203-216, and/or 235, and/or 243, and/or 259 with neutral or anionic amino acid showing enhanced resistance to oxidation followed by gene cloning into expression vector, transformation of strain-recipients Basillus with prepared recombinant DNAs, isolation and purification of the end product. EFFECT: improved method of protease preparing. 11 cl, 8 dwg