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2 252 958

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IPC

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Application

2000112540/13, 1998-10-23

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Start date

1998-10-23

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Actual filing date

1998-10-23

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Published

2005-05-27

(72)

Inventor

Poulouz Ajrookaran Dzh.Shellenberger Fol'KerKellis Dzhejms T. Ml.Pich KristianNehdkherni DzhoannNehjki Donal'D P.Kaldvell Robert M.Koll'Er Kehtrin D.

(73)

Holder

Dzhenenkor Internehshnl, Ink.

SUBTILISIN VARIANT (VARIANTS), DNA ENCODING THE SAME, EXPRESSION VECTOR, CLEANING COMPOSITION, ANIMAL FEED, COMPOSITION FOR CLOTH TREATMENT Russian patent published in 2005 - IPC

Abstract RU 2252958 C2

FIELD: enzymology, protein engineering, in particular enzyme with proteolysis activity.

SUBSTANCE: invention relates to new subtilisin variants obtained by substitution in amino acid sequence of wild-type enzyme following by certain charge alteration in corresponding site, namely negative charge increasing (positive charge decreasing) or vice versa. Subtilisin variants with the first-type substitutions have higher cleaning activities mainly in systems with law detergent concentrations. Subtilisin variants with the second-type substitutions have higher cleaning activities mainly in systems with high detergent concentrations. Variants being effective both in systems with low and high detergent concentration also are disclosed. New subtilisin variants are obtained by expression of DNA mutant sequence in cells of strain Bacillus. Subtilisin muteins of present invention are useful in cleaning compositions and composition for cloth treatment, as well as in animal feed additives.

EFFECT: enzyme of improved effectiveness.

18 cl, 4 dwg, 14 tbl, 2 ex

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RU 2 252 958 C2

Authors

Poulouz Ajrookaran Dzh.

Shellenberger Fol'Ker

Kellis Dzhejms T. Ml.

Pich Kristian

Nehdkherni Dzhoann

Nehjki Donal'D P.

Kaldvell Robert M.

Koll'Er Kehtrin D.

Dates

2005-05-27—Published

1998-10-23—Filed