FIELD: microbiology, biochemistry. SUBSTANCE: method of serine protease preparing involves cultivation of strain of alkaliphylleous Bacillus PB92 transformed with recombinant plasmid DNA pMAX-4 following by isolation and purification of the end product. Transformation of strain is carried out by strain treatment with lysozyme in alkaline medium at 20-37 C. Protoplast formed from lysozyme, combining of protoplast with recombinant plasmid DNA are carried out at the same temperature in the presence of reagent for mixing, regeneration of strain cells and growing of regenerated cells under selective conditions. EFFECT: improved method of serine protease preparing. 1 tbl
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