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2 269 572

(13)

(51)

IPC

C12N9/14(2006-01-01)

C12N15/57(2006-01-01)

C12N15/63(2006-01-01)

A23K1/165(2006-01-01)

(21) (22)

Application

2000112539/13, 1998-10-23

(24)

Start date

1998-10-23

(22)

Actual filing date

1998-10-23

(45)

Published

2006-02-10

(72)

Inventor

Shellenberger Fol'KerKellis Dzhejms T. Ml.Pich KristianNehdkherni DzhoannNehjki Donal'D P.Poulouz Ajrookaran Dzh.Koll'Er Kehtrin D.Kaldvell Robert M.Bak Andreh S.

(73)

Holder

Dzhenenkor Internehshnl, Ink.Dze Prokter Ehnd Gehmbl Kompani

MULTISUBSTITUTED PROTEASE (VARIANTS) Russian patent published in 2006 - IPC C12N9/14 C12N15/57 C12N15/63 A23K1/165

Abstract RU 2269572 C2

FIELD: biotechnology, light industry.

SUBSTANCE: invention relates to protease variants obtained by substitution in subtilisin amino acid sequence from Bacillus amyloliquefaciens, Baccilus subtilus, Baccilus licheniformis, and Baccilus lentus of amino acid residues in 103, 236 and/or 245 sites and in one ore more other sites followed by selection of mutants having improved washing action. DNA fragments encoding said protease variants and expression vector also are disclosed.

EFFECT: detergent compositions with improved washing action.

24 cl, 7 dwg, 5 tbl, 3 ex

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RU 2 269 572 C2

Authors

Shellenberger Fol'Ker

Kellis Dzhejms T. Ml.

Pich Kristian

Nehdkherni Dzhoann

Nehjki Donal'D P.

Poulouz Ajrookaran Dzh.

Koll'Er Kehtrin D.

Kaldvell Robert M.

Bak Andreh S.

Dates

2006-02-10—Published

1998-10-23—Filed