METHOD OF PREPARING THE TUMOR NECROSIS FACTOR, HUMAN TUMOR NECROSIS FACTOR Russian patent published in 1997 - IPC

FIELD: biotechnology, molecular biology. SUBSTANCE: tumor factor necrosis (TFN) is prepared by human cells HL-60 or PBLs cultivation followed by isolation and purification of the end product by the successive chromatography on glass beads, mono-Q-resin for rapid liquid chromatography and then by preparative electrophoresis on DS-polyacrylamide gel and PAAT-electrophoresis or liquid chromatography on C₄-reverse phase. The obtained human TNF shows capability to destroy or inhibit tumor cells and exhibits the following properties: molecular mass - 17000 D (assay by method using SDS-PAGE), isoelectric point - at 5.3, specific activity - 10 ml x U/mg protein. Human TNF is nonglycolized. Human TNF can be used in molecular biology, lymphokinesis, cytotoxic factors. EFFECT: improved method of preparing. 3 cl, 10 dwg, 1 tbl