FIELD: biotechnology, biochemistry. SUBSTANCE: method involves culturing the recombinant strain-producer E. coli SG 20050/pTNF 311 Δ. Cells are disrupted by ultrasonic oscillation and cell derbis are removed. Purification of the end product is carried out by successive chromatography on DEAE-cellulose in linear gradient of NaCl at pH 7.6 ± 0.1, on hydroxylapatite in linear gradient of potassium buffer at pH 6.9 ± 0.1 and on DEAE-cellulose again. EFFECT: simplified method, increased purity degree of recombinant tumor necrosis alpha-factor. 7 dwg, 4 tbl, 5 ex
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