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2 132 386

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Application

97110634/13, 1997-06-20

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Actual filing date

1997-06-20

(45)

Published

1999-06-27

(72)

Inventor

Zakabunin A.I.Baranovskaja G.A.Pustoshilova N.M.Majstrenko V.F.Gavrjuchenkova L.P.Gromova O.A.

(73)

Holder

Gosudarstvennyj Nauchnyj Tsentr Virusologii I Biotekhnologii

METHOD OF HUMAN RECOMBINANT GAMMA-INTERFERON PREPARING Russian patent published in 1999 - IPC

Abstract RU 2132386 C1

FIELD: biotechnology. SUBSTANCE: human recombinant gamma-interferon is prepared from insoluble inclusion-bodies of E. coli cells by disintegration of the strain-producer cells in buffer solution in the presence of zinc and copper salts, solubilization of the product with a mixture of urea and lithium chloride followed by chromatography purification on cation-exchanger COOH-Biochrom K-1. Cation-exchanger is a copolymer of methacrylic acid and pentaerythritol dimethacrylate. Method ensures to carry out easily the process of the isolation and purification of this biologically active substance in large-scale quantity. EFFECT: improved method of preparing, high purity of the end product. 2 cl, 1 dwg, 3 tbl, 4 ex

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RU 2 132 386 C1

Authors

Zakabunin A.I.

Baranovskaja G.A.

Pustoshilova N.M.

Majstrenko V.F.

Gavrjuchenkova L.P.

Gromova O.A.

Dates

1999-06-27—Published

1997-06-20—Filed

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