METHOD OF FOREIGN DNA INCORPORATION IN SPERMATOZOONS Russian patent published in 1997 - IPC

Abstract RU 2081914 C1

FIELD: genetic engineering. SUBSTANCE: before spermatozoons washing from the semen fluid their subjected for flotation. Spermatozoon suspension is incubated with DNA to be incorporated firstly at the room temperature in the presence of 1% DMSO, then DMSO concentration is increased to 3% and mixture is cooled to 0-(+4) C followed by its heating to 42 C. EFFECT: improved method of DNA incorporation. 2 cl, 1 tbl

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RU 2 081 914 C1

Authors

Kuznetsov Andrej Vadimovich

Kuznetsova Irina Vladimirovna

Naroditskij Boris Savel'Evich

Gol'Dman Igor' L'Vovich

Ehrnst Lev Konstantinovich

Dates

1997-06-20Published

1994-07-21Filed