FIELD: genetic engineering. SUBSTANCE: before spermatozoons washing from the semen fluid their subjected for flotation. Spermatozoon suspension is incubated with DNA to be incorporated firstly at the room temperature in the presence of 1% DMSO, then DMSO concentration is increased to 3% and mixture is cooled to 0-(+4) C followed by its heating to 42 C. EFFECT: improved method of DNA incorporation. 2 cl, 1 tbl
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Authors
Dates
1997-06-20—Published
1994-07-21—Filed