FIELD: veterinary medicine.
SUBSTANCE: invention concerns a method for capacitation of cryopreserved cattle spermatozoa for in vitro ovum fertilization in a completely chemically "defined" system. The method includes dbcAMF introduction into the protein-free Tyrode fertilization medium, instead of 10 μg/ml of heparin, at the concentration of 100 μM as the capacitating agent. The fertilization medium does not contain glucose, as well as any additives that activate spermatozoa.
EFFECT: increased efficiency of in vitro production of bovine embryos, can be used for research purposes.
2 tbl,1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF USING EXTRACELLULAR FOLICULAR FLUID VESICLES TO INCREASE HUMAN SPERMATOZOA MOBILITY IN VIRTUE FERTILIZATION PROGRAMS | 2022 |
|
RU2801339C1 |
| METHOD FOR PIG EMBRYONS GENERATION IN VITRO | 2007 |
|
RU2340177C1 |
| METHOD OF PRODUCTION OF SHEEP EMBRYOS in vitro | 2013 |
|
RU2525714C1 |
| METHOD OF TREATING MALE INFERTILITY WITH HIGH DNA FRAGMENTATION OF EJACULATORY SPERM CELLS | 2019 |
|
RU2685797C1 |
| METHOD OF PRODUCING CATTLE EMBRYOS IN VITRO | 2023 |
|
RU2818068C1 |
| METHOD FOR EXTRACORPOREAL CULTIVATING BOVINE OOCYTES | 2009 |
|
RU2410063C1 |
| METHOD FOR PREDICTION OF PREGNANCY RATE THROUGH IN VITRO FERTILISATION WITH SELECTIVE EMBRYO TRANSFER BY REAL-TIME PCR EVALUATION OF MOLECULAR GENETIC GAMETE PROFILE | 2014 |
|
RU2550965C1 |
| METHOD FOR SPERM CELL SELECTION FOR EXTRACORPOREAL FERTILISATION | 2013 |
|
RU2535359C1 |
| METHOD FOR DIFFERENTIATION OF LIVING SPERM CELLS USING DIAGNOSTIC CULTURE MEDIUM IN CASE OF ABSOLUTE ASTHENOZOOSPERMIA WITHIN IN VITRO FERTILIZATION | 2015 |
|
RU2603084C2 |
| METHOD FOR PRODUCTION OF TRANSGENIC CHICKENS BY MEDIATED TRANSGENESIS THROUGH CONVENTIONAL ARTIFICIAL INSEMINATION | 2011 |
|
RU2517731C2 |
