FIELD: virology. SUBSTANCE: invention relates to producing viral antigen of spring-summer meningoencephalitis (FSME) by culturing virus FSME in cell cultures depending on the surface of permanent cellular strain, preferably, vero-cellular strain ATCC CCL-81. Method involves infection with virus FSME and cells were maintained in nonlytic serumless system as bound with carriers to provide antigen formation. Then antigen-containing medium is separated from cells bound with carriers by the known method followed by concentration, inactivation and purification and treated to obtain galenic preparation. EFFECT: improved method of preparing. 6 cl
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Authors
Dates
1997-06-27—Published
1990-12-21—Filed