METHOD OF VACCINE PREPARING, A METHOD OF CELL-FREE VACCINE PREPARING, A METHOD OF CELLS CULTURING, A NUTRIENT MEDIUM Russian patent published in 1999 - IPC

FIELD: virology, vaccines. SUBSTANCE: invention relates to preparing live attenuated vaccine based on varicella-zoster and shingles extracellular virus. Method involves culturing monolayer cultures in medium enriched with lipid additions that ensures to increase the yield of viral plaque-forming units and viral antigen. Using standard flasks for culturing cell density achieves 500 000 cells/cm². and use of an optimal conditions during culturing ensures to increase viral vaccine producing. Invention can be used in biotechnology and immunology. EFFECT: improved method of vaccine preparing. 12 cl, 5 dwg, 6 tbl, 20 ex