FIELD: veterinary science; biotechnology.
SUBSTANCE: invention relates to veterinary biotechnology and virology. Described is a method for producing a culture inactivated rabies virus vaccine, involving continuous perfusion culturing of kidney cell line of a newborn Syrian hamster BHK-21 using filtration system with variable tangential flow and regulation of perfusion rate, their infection with rabies virus, virus replication and inactivation of virus-containing suspension with subsequent production of rabies virus glycoprotein.
EFFECT: invention enables to obtain an inactivated rabies virus vaccine in industrial volumes with a high concentration of virus-infected cells, a high titre of infectious activity of the virus and high immunogenicity of the obtained vaccine.
5 cl, 1 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| ENA-21/13-13-FINITE MONOLAYER-SUSPENSION CELL SUBLINE OF KIDNEY OF NEW-BORN SYRIAN HAMSTER, INTENDED FOR REPRODUCTION OF FOOT-AND-MOUTH DISEASE VIRUS AND RABIES VIRUS | 2014 |
|
RU2553552C1 |
| METHOD OF PRODUCTION OF ANTIRABIC VACCINE | 2013 |
|
RU2538617C2 |
| BHK-21/SUSP/ARRIAH - CONTINUOUS SUSPENSION SUBLINE OF NEWBORN SYRIAN HAMSTER KIDNEY CELLS, INTENDED FOR REPRODUCTION OF FOOT-AND-MOUTH DISEASE VIRUSES, RABIES, PARAINFLUENZA-3, AUJESZKY'S DISEASE IN PRODUCING ANTIVIRAL VACCINES, AS WELL AS FOR MAKING DIAGNOSTIC AND PREVENTIVE VETERINARY BIOPREPARATIONS | 2019 |
|
RU2722671C1 |
| SERUM-FREE ENVIRONMENT “VNIIZZH” FOR THE CULTIVATION OF VNK-21 KIDNEY CELLS OF THE SYRIAN HAMSTER AND THE PRODUCTION OF IMMUNOGENIC COMPONENTS OF CULTURE-BASED VIRUSES OF FOOT-AND-MOUTH DISEASE AND RABIES FOR THE MANUFACTURE OF VACCINES | 2021 |
|
RU2770814C1 |
| BHK-21/13-02-TRANSPLANTABLE MONOLAYER-SUSPENSION SUBLINE OF NEWBORN SYRIAN HAMSTER KIDNEY CELLS, INTENDED FOR FMD VIRUS AND RABIES VIRUS REPRODUCTION | 2005 |
|
RU2614074C1 |
| METHOD FOR DETERMINING THE OPTIMAL TIME OF REPRODUCTION OF THE RABIES VIRUS IN THE CELLS OF THE KIDNEY LINE OF A NEWBORN SYRIAN HAMSTER BHK-21/SUSP/ARRIAH FOR THE MANUFACTURE OF ANTI-RABIES VACCINES USING THE ANALYSIS OF TRANSFORMATIONAL CHANGES IN CELLULAR DNA BY FLOW CYTOMETRY | 2022 |
|
RU2796497C1 |
| BHK-21/13-02 - TRANSPLANTABLE MONOLAYER-SUSPENSION SUBLINE OF NEWBORN SYRIAN HAMSTER KIDNEY CELLS DESIGNATED FOR REPRODUCTION OF FOOT-AND-MOUTH VIRUS AND RABIES VIRUS | 2005 |
|
RU2300562C2 |
| ANTIRABIC INACTIVATED EMULSION CULTURE VACCINE FOR PROPHYLACTIC IMMUNIZATION OF DOMESTIC CARNIVORES AND FARM ANIMALS | 2019 |
|
RU2722868C1 |
| ANTI-RABIES VACCINE FOR ORAL IMMUNISATION OF WILD CARNIVOROUS ANIMALS AND METHOD OF ITS OBTAINING | 2010 |
|
RU2440139C1 |
| CULTURAL INACTIVATED EMULSION VACCINE AGAINST FOOT AND MOUTH DISEASE FROM O NO. 2620/ORENBURGSKY/2021 STRAIN OF O/ME-SA/IND-2001E GENOTYPE | 2023 |
|
RU2815537C1 |
