FIELD: biotechnology, microbiology, analytical analysis. SUBSTANCE: method involves interaction of an examined substance aqueous solution with bioindicator, recording oxygen concentration change in medium before and after interaction mentioned above and determination by oxygen concentration change presence and analyzed substance concentration. Interaction is carried out in saline medium using microorganism cells as bioindicator, in part, cells of the strain Brevibacterium species VKPM B-4987 or the strain Pseudomonas pseudoalcaligenes VKPM B-4418, or the strain Rhodococcus rhodochrous VKPM S-926. Method is used for detection and determination of aliphatic acid with short hydrocarbon chain and their amides in aqueous solutions. EFFECT: improved method of analysis. 3 cl, 4 tbl
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