METHOD DETERMINATION OF BIOCATALYST ENZYMATIC ACTIVITY Russian patent published in 1998 - IPC

FIELD: biochemistry, enzymology. SUBSTANCE: method involves preparing standard and examined samples, carrying out an enzymatic reaction with the latter, determination of an optical density of samples after reaction termination, evaluation of an enzymatic activity of biocatalyst by alternation of optical density value. After enzymatic reaction termination followed by recording optical density values of both samples the latter is subjected for by electric field and in the process of this treatment optical value of samples were recorded again. Ratio of optical values of corresponding samples were calculated before and after effect by electric field and the presence of enzymatic activity in biocatalyst is estimated by comparison. Method can be used in biotechnology and medicine for analysis of microorganisms, enzymes and so on. EFFECT: improved method of assay. 6 dwg