FIELD: biotechnology, biochemistry, enzymology. SUBSTANCE: cattle frozen organs were homogenized, and the protease basic inhibitor is extracted with an acid aqueous solution at pH = 1-3. The obtained extract is fractionated and concentrated by ultrafiltration at tangential flow on the hollow fibers (firstly at pore size is 15-50 kDa and then at 5 kDa). Final purification is carried out by cation-exchange chromatography. Yield and specific activity of the end product were increased by 8- and 10.8-fold, respectively. Invention can be used in medicine, cellular and molecular biology. EFFECT: increased yield and specific activity, simplified technology, decreased cost. 2 cl
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