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(19)

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2 027 759

(13)

C1

(51)

IPC

(21) (22)

Application

5025578/13, 1992-02-03

(22)

Actual filing date

1992-02-03

(45)

Published

1995-01-27

(72)

Inventor

Pak V.N.Ofitserov V.I.

(73)

Holder

Pak Vladimir Nikolaevich

METHOD OF HYALURONIDASE PREPARING Russian patent published in 1995 - IPC

Abstract RU 2027759 C1

FIELD: biotechnology. SUBSTANCE: frozen testicles of slaughter animals were homogenized, and hyaluronidase is extracted with diluted acetic acid. Extract is separated by centrifugation, prepared extract is concentrated in tangential flow, part of ballast protein is removed by precipitation with polyethylene glycol at concentration 5-25 wt.-% following by cation-exchange chromatography. Product is used for medicinal preparations preparing. EFFECT: increased specific activity of the end product, purification of preparation from bovine serum albumin. 2 cl

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RU 2 027 759 C1

Authors

Pak V.N.

Ofitserov V.I.

Dates

1995-01-27—Published

1992-02-03—Filed

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