FIELD: medicine, clinical immunology. SUBSTANCE: invention relates to determination of monocytes functional state by reaction of phagocytosis of the opsonized zymosan particles. Reaction is carried out on glass with the stuck cells obtained from mononuclear cells suspension isolated from the human venous blood using density gradient in ficoll-verografin. Normal state of blood monocytes is determined by amount cells phagocytizing zymosan, 94.8 ± 4.7%. Method ensures to evaluate functional state of blood monocytes in healthy human and its disorder at pathology. EFFECT: improved method of evaluation.
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Authors
Dates
1997-09-10—Published
1994-05-05—Filed