METHOD OF PREPARING NUCLEIC ACID FROM BACTERIA Russian patent published in 1997 - IPC

Abstract RU 2091491 C1

FIELD: biotechnology, microbiology. SUBSTANCE: biomass of bacterium Methylococcus capsulatus is extracted with an aqueous-alkaline or an aqueous-saline solution at pH = 7.5, not above. At the first stage RNA is separated (at 85-90 C) and at the second stage a mixture of RNA and DNA is separated at pH = 8.6-9.0 followed by their separation by the known methods. EFFECT: high purity of DNA and RNA.

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RU 2 091 491 C1

Authors

Krylov I.A.

Panfilov V.I.

Volkova N.V.

Manakov M.N.

Dates

1997-09-27Published

1995-07-14Filed