Patenton — panteon of patents

(19)

RU

(11)

2 101 354

(13)

C1

(51)

IPC

(21) (22)

Application

95101495/13, 1995-01-31

(22)

Actual filing date

1995-01-31

(45)

Published

1998-01-10

(72)

Inventor

Abgarjan A.G.Majskij V.G.Eremenko E.I.

(73)

Holder

Stavropol'Skij Nauchno-Issledovatel'Skij Protivochumnyj Institut

METHOD OF BACILLUS ANTHRACIS NUCLEIC ACID PREPARING Russian patent published in 1998 - IPC

Abstract RU 2101354 C1

FIELD: biochemistry, biotechnology, bacteriology. SUBSTANCE: biomass is obtained at preliminary additional culturing microbe cells Bacillus anthracis under condition of the sealed flasks containing culture. Biomass is treated with lysozyme in presence of EDTA at concentration 20 mg/1 g biomass. The obtained suspension is frozen three times in liquid nitrogen followed by thawing at 60 C. Freezing time is 40-60 s. Then additional lysis with tris-SDS-buffer is carried out. Nucleic acids were purified with phenol and precipitated with cold ethanol. EFFECT: improved method of nucleic acid preparing. 3 tbl

Similar patents RU2101354C1

Title Year Author Number
METHOD FOR RESTORING BIOSYNTHESIS ABILITY OF PEST MICROBE CAPSULE ANTIGEN 1999
  • Zajtsev A.A.
  • Evchenko Ju.M.
  • Shchedrin V.I.
  • Tsareva N.S.
 RU2155962C1
METHOD OF ANTHRAX BACTERIOPHAGE GAMMA A-26 PREPARING 1999
  • Solodovnikov B.V.
  • Tjumentseva I.S.
  • Efremenko V.I.
  • Afanas'Ev E.N.
 RU2171842C2
METHOD FOR ISOLATING OF COCCIDIOIDES IMMITIS DNA FOR POLYMERASE CHAIN REACTION (PCR) 2005
  • Tkachenko Galina Aleksandrovna
  • Grishina Marina Anatol'Evna
  • Savchenko Sergej Sergeevich
  • Antonov Valerij Alekseevich
 RU2295569C1
METHOD FOR ISOLATING CHROMOSOMAL DNA FROM BACTERIAL CELLS OF PLAGUE PATHOGEN, YERSINIA PESTIS 2002
  • Savostina E.P.
  • Popov Ju.A.
  • Korsukov V.N.
 RU2232818C1
METHOD OF ANTHRAX BACTERIOPHAGE "GAMMA" CULTURING 1996
  • Tsygankova O.I.
  • Solodovnikov B.V.
 RU2133273C1
METHOD OF BACTERIAL CULTURE PHAGE TYPIFYING 1997
  • Savel'Ev V.N.
  • Kal'Naja E.A.
  • Evchenko Ju.M.
  • Kruchinin A.D.
 RU2137838C1
METHOD OF INDICATION OF MICROORGANISMS 1999
  • Efremenko V.I.
  • Tjumentseva I.S.
  • Zhilchenko E.B.
  • Markova T.V.
  • Sokolova I.A.
  • Kastornaja M.N.
  • Abgarjan A.G.
  • Urusbambetov Zalim Geri Khasanovich
  • Mezentseva O.N.
  • Eremenko E.I.
  • Brjukhanov A.F.
  • Afanas'Ev E.N.
  • Son D.D.
 RU2165081C2
METHOD OF COUPLED ESTIMATION OF FUNCTIONAL ACTIVITY OF PERIPHERAL BLOOD NEUTROPHILS AT BRUCELLOSIS PATHOGEN EFFECT 1997
  • Logvinenko O.V.
  • Ljamkin G.I.
  • Golub' O.G.
  • Rudnev S.M.
 RU2133471C1
METHOD OF ANTIGEN PREPARING FOR BRUCELLOSIS DIAGNOSIS 1997
  • Ljapustina L.V.
  • Ljamkin G.I.
  • Dal'Vadjants V.G.
  • Taran I.F.
 RU2133470C1
METHOD FOR ISOLATION OF HETEROGENEOUS ANTIGENS SIMILAR WITH ANTIGENS OF MICROORGANISMS OF GENUS BRUCELLA AND HUMAN PARENCHYMATOUS TISSUES 2001
  • Efremenko V.I.
  • Kanteeva E.A.
  • Borzdova I.Ju.
  • Zarubin V.V.
  • Shevchenko O.A.
 RU2215295C2

RU 2 101 354 C1

Authors

Abgarjan A.G.

Majskij V.G.

Eremenko E.I.

Dates

1998-01-10—Published

1995-01-31—Filed

read Similar patents