FIELD: biotechnology, microbiological industry. SUBSTANCE: biomass is grown on nutrient medium containing carbon, nitrogen and necessary salts source and stimulator of cell growth. Stimulator: N-tris-(hydroxyethyl)-ammonium salt of para-chlorophenylthioacetic acid. Additional stimulating agent is complex preparation gibbersib at concentration I·IO-12 - I·IO-6 % % obtained from cultural fluid forming at fungus Fusarium moniliforma culturing. Method ensures to increase biomass yield by 10-15% of carbon source used and simultaneous increase of protein content in biomass. Consumption of para-chlorophenylthioacetic acid N-tris-(hydroxyethyl)-ammonium salt is decreased by IO3 - IO6-fold. EFFECT: improved method of biomass preparing, increased yield, decreased reagent consumption.
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Authors
Dates
1997-10-20—Published
1992-04-08—Filed